ESPEN 2022 Abstract Submission
Topic: Obesity and the metabolic syndrome
Abstract Submission Identifier: ESPEN22-ABS-1916
FECAL BILE ACID AND GUT MICROBIOTA PROFILE AFTER ROUX-EN-Y GASTRIC BYPASS IN OBESE WOMEN WITH TYPE 2 DIABETES REMISSION: IS THERE A RELATIONSHIP?
D. C. Fonseca*, 1, A. P. Aguiar Prudêncio 1, C. Cardinelli 1, N. M. Machado 1, J. T. M. Alves 1, 2, B. A. M. Ferreira 1, P. Sala 3, G. D. R. Fernandes 4, R. Torrinhas 1, D. L. Waitzberg 1
1Gastroenterologia, LABORATORY OF NUTRITION AND METABOLIC SURGERY OF THE DIGESTIVE SYSTEM, LIM 35. DEPARTMENT OF GASTROENTEROLOGY, HOSPITAL DAS CLINICAS HCFMUSP, FACULDADE DE MEDICINA, UNIVERSIDADE DE SAO PAULO, SAO PAULO., São Paulo, 2Medical Clinical Nutrition, Hospital Sirio Libanes, Brasilia, 3Nutrition, Sao Camilo University, São Paulo, 4Instituto Rene Rachou - Fiocruz Minas, Belo Horizonte, Brazil
Rationale: Bile acids (BAs) are associated to type 2 diabetes modulation. The gut microbiota (GM) transforms primary BAs into secondary BA, key players in T2DM remission after bariatric surgery.
Methods: We evaluated fecal BAs, GM profile and it’s relation with T2DM remission in 20 obese women before and 3 months after Roux-en-Y gastric by-pass surgery (RYGB). Fecal BAs were determined by target metabolomic analysis (liquid chromatography coupled to mass spectrometry). GM was assessed by Illumina V4 16S rRNA gene sequencing. Appropriate statistics tests were done with 5% significant level. T2DM remission was defined by the ADA criteria. Data were colletect from the SURMetaGIT cohort study, approved by local ethics committee, and registered at Clinical Trials (NCT01251016).
Results: All patients achieved glycemic improvement at 3 months post-op. However, patients who achieved partial remission (PR) showed no changes in fecal BAs and GM taxas (p>0.05), while patients with total remission (TR) showed changes in 12 fecal BAs and 11 bacterial taxa after RYGB (p<0.05). Significant correlations between secondary fecal BAs and GM taxas species were observed only in TR group. Strong and positive correlations were found between Feacalibacteruim prausnitzii and the acids glycochenodeoxycholic (Rho= 0.738 ; p= 0.046), taurochenodeoxycholic (Rho= 0.753; p= 0.031), taurodeoxycholic (Rho=0.855; p=0.007); taurolithocholic (Rho=0.783; p=0.017). Streptococcus salivarius was positively correlated to taurochenodeoxycholic acid (Rho=0.707 ; p=0.022); Eubacterium eligens was negatively correlated with deoxycholic acid (Rho= -0.833; p=0.015), glycodeoxycholic acid (Rho= -0.624; p=0.040).
Conclusion: RYGB was followed by changes in the profile of GM and BAs, with strong correlations between GM species and secondary BA only in TDM2 total remission patients.
Disclosure of Interest: None Declared
Keywords: Bariatric Surgery, Diabetes Mellitus, Gut microbiota, Metabolites of intestinal microbiota