ESPEN 2013 - Late breaking abstract submission
Late Breaking Abstract
ESPEN13-1806
B-HYDROXY-B-METHLYBUTYRATE (HMB) MODULATES CIRCULATING BIOMARKERS IN OLDER ADULTS ON EXTENDED BEDREST (BR)
S. L. Pereira 1,*G. J. Davis 2M. Luo 1S. H. Gawel 2R. Chandran 2N. E. Deutz 3R. R. Wolfe 4N. K. Edens 1
1Abbott Nutrition, Ohio, 2Abbott Diagnostics, Illinois, 3Geriatrics, Texas A&M University, Texas, 4Geriatrics, University of Arkansas for Medical Sciences, Arkansas, United States
Rationale: Short term BR due to hospitalization is a major contributor to loss of muscle and functional decline in older adults. Ca-β-hydroxy-β-methlybutyrate (HMB) at 3g/d was recently found to prevent muscle loss over BR in healthy older adults. To investigate the mechanism of action of HMB, changes in serum biomarkers from older adults on BR were measured in HMB-supplemented versus Control subjects.
Methods: Fasted serum samples were obtained from eighteen healthy older adults (age 60-76 y) who were supplemented with either placebo control (n=8) or HMB (n=10) over 10 days of bedrest. Samples taken at the start and at the end of BR were analyzed for biomarkers using multiplexed immunoassay array. Of the 187 markers analyzed, 63 were excluded from analysis due to low detection levels in ≥ 30% of the subjects. Individual univariate dependent t-tests were performed on 124 markers in control group and markers with significant changes were further analyzed in HMB group by univariate ANCOVA (using a Bonferonni adjusted p-value of 0.0038).
Results: Thirteen markers were significantly changed over BR (p<0.05) in the Control group. 10 of the 13 markers were significantly changed after BR between the control and HMB groups, while controlling for pre-BR baseline differences. Physiological pathways associated with these markers link to metabolic abnormalities due to immobilization.
Table: Select marker changes over BR (*p<0.0001)
Markers (ng/ml) |
Control |
HMB
| ||||
|
|
Pre-BR |
Post-BR |
%Δ |
Pre-BR |
Post-BR |
%Δ* |
|
IGF-BP2 |
102.9±44.3 |
72.3±26.2 |
-25±8 |
127.9±17 |
107±15.2 |
-16±4 |
|
Myoglobin |
72.4±48.6 |
40.8±20.6 |
-38±8 |
49.3±5 |
39.1±4 |
-19±6 |
|
Matrix metalloprotease-1 |
10.4±7.5 |
12.1±8.2 |
28±8 |
11.3±3.5 |
13.5±4.4 |
15±7 |
Data represents Mean ± SEM
Conclusion: HMB positively modulates markers associated with metabolic abnormalities during BR in older adults.
Disclosure of Interest: S. Pereira Shareholder of: AbbottG. Davis Shareholder of: AbbottM. Luo Shareholder of: AbbottS. Gawel Shareholder of: AbbottR. Chandran Shareholder of: AbbottN. Deutz: None DeclaredR. Wolfe Grant / Research Support from: AbbottN. Edens Shareholder of: Abbott
Keywords: Bedresthospitalized elderly