ESPEN 2013 - Late breaking abstract submission

Late Breaking Abstract

ESPEN13-1833

THE INFLUENCES OF OBSTRUCTIVE JAUNDICE ON PEYER’S PATCH LYMPHOCYTE NUMBERS AND SUBPOPULATIONS IN MICE

T. Moriya ^1,*K. Fukatsu ²M. Noguchi ³K. Okamoto ¹A. Kimura ¹S. Aosasa ¹K. Sasaki ⁴D. Saitoh ³K. Hase ¹J. Yamamoto ¹

¹Surgery, National Defense Medical College, Tokorozawa, ²Surgical center, University, Tokyo, ³Division of Traumatology, National Defense Medical College Research Institute, ⁴Surgery, Sasaki Memorial Hosp., Tokorozawa, Japan

Rationale: Obstructive jaundice(OJ) has been demonstrated to be one of the major causes of postoperative infectious complications.OJ results in release of pro-inflammatory cytokines, impairs the enterohepatic circulation and induces bacterial translocation, which play key roles in infectious complications. One reason for these phenomena in jaundiced patients is loss of intestinal mucosa barrier function. Although OJ-related changes in gut morphology and permeability have been demonstrated, the influence of OJ on Gut Associated Lymphoid Tissue(GALT), the center of mucosal immunity, is not fully understood.This study was designed to examine the effect of OJ on Peyer’s patches(PPs), inductive sites of GALT, foscuing on lymphocyte cell numbers and subpopulations in the PPs of mice.

Methods: Twenty-four male ICR mice were randomized to two groups; Control(n=10) and OJ(n=14). In the Control group,a simple laparotomy was performed,while in the OJ group,the common bile ducts were ligated with 5-0 silk.On day 7, after the whole small intestine had been harvested, lymphocytes from PPs were isolated and counted. Their phenotypes were analyzed with flowcytometry (CD4,CD8,αβTCR,γδTCR,B220).

Results: Ligation of the bile duct resulted in significant body weight loss as compared with the Control.Total lymphocyte numbers in PPs were significantly lower in the OJ than in the Control group.The percentage of B220 positive lymphocytes was also significantly lower in the OJ than in the Control group.

Conclusion: OJ decreased body weight and lymphocyte cell numbers in PPs.The present data suggest OJ to impair mucosal immunity, making patients more susceptible to bacterial infection.

Disclosure of Interest: None Declared

Keywords: None